Intensity of feulgen staining of rat liver nuclei fixed. Feb 04, 2016 tincion diferencial clasifica bacterias en dos grandes grupos. The results presented in this paper indicate that following fixation of rat liver in either 40% wv or 10% formalin solution, feulgen staining is far greater in the tissue fixed in the former fixative as compared with the same fixed in the latter. Schiff reagent but is a solution of pure pararosaniline in a concentration of 0. A possible mode of action of formalin towards fixation and subsequent feulgen staining is suggested. Download as pptx, pdf, txt or read online from scribd. Feulgen stain is a staining technique discovered by robert feulgen and used in histology to identify chromosomal material or dna in cell specimens. Youre covered by the ebay money back guarantee if you receive an item escrime medievale is not as described in the listing. Doc tecnica histologica y microscopia diana paola iniguez.
The chemistry of the feulgen reaction and related histo core. In some instances, the result is invalid, whereby the test should be repeated. The feulgen reaction, proposed by feulgen and rossenbeck 75 years ago, is commonly regarded as one of the oldest and most specific reactions in histochemistry. Tincion acido alcohol resistente fundamento unquotable ruddie timken ball fundamento tincion alcohol acido resistente. Coloracion ziehl neelsen download as powerpoint presentation. Results from the xpert mtbrif assay indicate whether or not mtbc was detected in the sample. Today, one of the most important applications of the feulgen reaction is quantifying dna amounts. It depends on acid hydrolysis of dna, therefore fixating agents using strong acids should be avoided. Discover everything scribd has to offer, including books and audiobooks from major publishers. Retire as amostras prefixadas e laveas em agua destilada. Jul 01, 2017 proposed nearly a hundred years ago by robert feulgen and heinrich rossenbeck 1924, the feulgen reaction continues to offer an opportunity for many research insights in fields such as cytogenetics, cell biology and analytical cytopathology hardie et al. Feulgen stain is a staining technique discovered by robert feulgen and used in histology to.
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